Arlene may refer to:
- Arlene (name), a feminine name, also spelled Arleen
- Arlene (Garfield), a fictional character in Garfield
- "Arlene" (song), the 1985 debut single by American country music artist Marty Stuart
- Arlene, a Beanie Baby cat produced by Ty, Inc.
- Arlene (The X-Files), a fictional character in The X-Files
- Hurricane Arlene, the name of several tropical storms in the Atlantic Ocean