Zoe Tuckwell-Smith was born in Sydney. Shortly after, her parents returned to Bangladesh where they were working at the time. Tuckwell-Smith's parents divorced when she was five years old and she was raised by her mother in Sydney. Tuckwell-Smith visited her father during her school holidays. Tuckwell-Smith enjoyed acting from a young age and said "I've always been a dreamer – imaginative and creative. As a child I loved putting on plays and making up stories to perform for my parents or my toys!" After finishing high school, Tuckwell-Smith was accepted into the National Institute of Dramatic Art (NIDA) and graduated three years later.
In 2004, Tuckwell-Smith appeared in Six Pack, All Saints and The Cooks. She went on to appear in Home and Away and In Search of Paradise. Tuckwell-Smith was cast in Stupid Stupid Man in 2007. The following year, the actress made appearances in The Cut and The Strip.
As well as television, Tuckwell-Smith has appeared in various feature films, including Son of the Mask (2004), Gone (2005), Cactus (2008) and Primal (2009). In 2010, Tuckwell-Smith also guested in Short Stack's video clip for "Planets".
Tuckwell-Smith has been in a relationship with actor Damon Gameau since 2009. The couple were introduced to each other by fellow actor Gyton Grantley, while they were both in East Timor. Tuckwell-Smith and Gameau announced that they were expecting their first child in June 2013. Tuckwell-Smith gave birth to the couple's daughter in November 2013.
|2004||Son of the Mask||Mrs. Babcock||First film role|
|2004||Six Pack||Charlie Butterfield||First television role|
|2004||All Saints||Elle Campbell -||Season 7 Episode 28|
|2005||Home and Away||Various characters|
|2006||In Search of Paradise||Nicki|
|2007||Stupid Stupid Man||Gwen|
|2011–2014||Winners & Losers||Bec Gilbert||Leading role|