Gherardini may refer to :
- Alessandro Gherardini (1655–1723) Italian painter of Baroque Florence.
- Maurizio Gherardini (b. 1955) Italian sportsman, currently vice president and an assistant general manager for the Toronto Raptors NBA club.
- Lisa Gherardini (1479–1542) woman depicted in the Mona Lisa painting.
- Gherardini family of Montagliari, aristocratic family of Florence.
- Stefano Gherardini (1695–1755) Italian genre painter
- Tommaso Gherardini (1715–1797) Italian painter of late 18th-century Florence