Josefine may refer to:
- Josefine Balluck (1908–1984), Austrian actress with dwarfism, played a Munchkin in "The Wizard of Oz"
- Josefine Cronholm (born 1971), Swedish jazz vocalist
- Josefine Engström, Swedish ski-orienteering competitor and World Champion
- Josefine Lindstrand, Swedish singer who was born in Örebro in 1981
- Josefine Öqvist (born 1983), female footballer for Linköpings FC and the Swedish national team
- Josefine Preuß (born 1986), German actress
- Josefine Ridell (born 1997), Swedish singer in the 2010 Junior Eurovision Song Contest